Summary: Researchers have discovered a two-pronged approach to restore myelin on regenerated axons in a mouse model of optic nerve damage. The findings have positive implications for the treatment of multiple sclerosis.

Source: Children’s Hospital of Boston

Loss of myelin—the fatty substance that surrounds the axons of nerve cells—is one of the reasons nerve cells fail to recover after injury and in some diseases. Myelin acts like insulation, covering the long axon threads that enable high-speed communication between neurons. Without myelin, the neurons may not be able to coordinate well, leading to less than optimal function.

In new research from the laboratory of Zhigang He, Ph.D., of the F.M. Kirby Neurobiology Center, scientists discovered a two-pronged approach to restore myelin on regenerated axons in a mouse model of optic nerve injury. Their findings may have implications for disease associated with myelin loss, like multiple sclerosis (MS).

“We believe that this work represents an important step towards functional restoration of cells in the adult central nervous system,” says He.

Axons fail to remyelinate after injury

In earlier research, He’s lab discovered several treatments that could promote the regeneration of damaged axons in the optic nerve, but do not restore nerve function. The reason? These regenerated axons are not myelinated. In this new study, published in Neuron, He explains why those axons fail to remyelinate after injury.

In the adult brain, myelination is carried out by cells called oligodendrocyte precursor cells (OPCs).

“We found that in injured optic nerves, OPCs fail to differentiate into mature myelination-competent oligodendrocytes,” says He, meaning they do not develop into cells capable of producing myelin and functioning normally.

He’s team discovered two reasons why. The first is that OPCs in injured nerves produce a protein known as GPR17, which blocks the first step of OPC differentiation into mature cells. Second, inflammatory cells in the injured nerves interfere with another step of OPC differentiation.

Combination treatment restores myelin

After testing a set of available compounds, co-first author Jing Wang, Ph.D., of the He lab, discovered that montelukast, an anti-inflammatory used for treatment of asthma and seasonal allergies, blocked development (or action) of GPR-17. Some axon remyelination was restored but only in about approximately 15 percent of treated nerve cells.

However, myelination rates were boosted significantly after removing immune cells, called microglia, from the damaged nerve cells with a drug called PLX3397. By itself, PLX3397 increased remyelination in 21 percent of axons. In combination with montelukast, the combination lead to remyelination in about 60 percent of damaged axons.

Typically, microglia act as scavengers in the central nervous system, looking for damaging inflammatory cells and infectious compounds and removing them from the site of the injury. However, in this case, the presence of microglia stopped the OPCs from developing into normal oligodendrocytes. Further studies with microglia showed that when they were removed later after injury—in this case two weeks after injury to the optic nerve—OPC development followed more normally.

In the adult brain, myelination is carried out by cells called oligodendrocyte precursor cells (OPCs). Image is in the public domain

“In a mice model of optic nerve injury, we found that when we combined treatment of montelukast and removing microglia later after injury, the majority of axons could be remyelinated,” He says.

Relevance to multiple sclerosis

See also

This shows a brain

In the advanced stage of multiple sclerosis (MS), injured nerves suffer from myelination failure. While this research was not conducted in a MS mouse model, the fact that He’s team discovered a way to remyelinate regenerated axons is encouraging since no currently available MS treatments currently work by rebuilding the myelin sheath.

“It is similar to what we see in the optic nerve after injury, “says He. “Both suffer from myelination failure so we believe our results could have some implications for new MS treatments, particularly for progressing MS.”


About this neurology research news

Source: Children’s Hospital of Boston
Contact: Press Office – Children’s Hospital of Boston
Image: The image is in the public domain

Original Research: Closed access.
“Robust Myelination of Regenerated Axons Induced by Combined Manipulations of GPR17 and Microglia” by Jing Wang et al. Neuron


Abstract

Robust Myelination of Regenerated Axons Induced by Combined Manipulations of GPR17 and Microglia

Highlights

  • Proliferated OPCs exhibit differentiation blockade in injured optic nerves
  • GPR17 inhibition promotes OPC differentiation
  • Chronically activated microglia prevent oligodendrocyte maturation
  • Co-manipulation of GPR17 and microglia promotes robust myelination

Summary

Myelination facilitates rapid axonal conduction, enabling efficient communication across different parts of the nervous system. Here we examined mechanisms controlling myelination after injury and during axon regeneration in the central nervous system (CNS). Previously, we discovered multiple molecular pathways and strategies that could promote robust axon regrowth after optic nerve injury. However, regenerated axons remain unmyelinated, and the underlying mechanisms are elusive. In this study, we found that, in injured optic nerves, oligodendrocyte precursor cells (OPCs) undergo transient proliferation but fail to differentiate into mature myelination-competent oligodendrocytes, reminiscent of what is observed in human progressive multiple sclerosis. Mechanistically, we showed that OPC-intrinsic GPR17 signaling and sustained activation of microglia inhibit different stages of OPC differentiation. Importantly, co-manipulation of GPR17 and microglia led to extensive myelination of regenerated axons. The regulatory mechanisms of stage-dependent OPC differentiation uncovered here suggest a translatable strategy for efficient de novo myelination after CNS injury.



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